Diagnosis of plant viruses by nucleic acid hybridization

8.5 Detecting Nucleic Acid Hybridization

The double-helical structure of nucleic acids, in which one strand binds specifically to its exact complement, could not have been designed to be more useful for the study of biology. The ease of designing assays based on this feature is responsible for the incredibly fast pace that has characterized molecular biology research since its inception. With the incorporation of fluorescence technolo...

Nucleic Acid Hybridization – General Aspects

Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescence in situ hybridization method

Bacterial vaginosis (BV) is one of most common vaginal infections. However, its diagnosis by classical methods reveals low specificity. Our goal was to evaluate the accuracy diagnosis of 150 vaginal samples with research gold standard methods and our Peptide Nucleic Acid (PNA) probes by Fluorescence in situ Hybridization (FISH) methodology. Also, we described the first PNA-FISH methodology for ...

Optimizing the specificity of nucleic acid hybridization.

The specific hybridization of complementary sequences is an essential property of nucleic acids, enabling diverse biological and biotechnological reactions and functions. However, the specificity of nucleic acid hybridization is compromised for long strands, except near the melting temperature. Here, we analytically derived the thermodynamic properties of a hybridization probe that would enable...

Molecular Diagnosis of Plant Viruses

Makkouk, K.M. and S.G. Kumari. 2006. Molecular Diagnosis of Plant Viruses. Arab J. Pl. Prot. 24: 135-138. Proper virus identification is always the key in developing appropriate practical solutions to manage plant virus diseases. Recent advances in biotechnology and molecular biology have played a significant role in the development of rapid, specific and sensitive diagnostic tests. The use of ...